Long term consequences of bilateral cavernous crush injury in normal and diabetic rats: a functional study
BAUS ePoster online library. Castiglione F. 06/23/21; 319042; p13-4
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Fabio Castiglione
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Introduction: A recent statement from the European Society for Sexual Medicine has highlighted the limits of the rat model for nerve sparing radical prostatectomy like the use of young rats without comorbidities and the early evaluation of the erectile function (EF).
The aim of this study is to evaluate the long-term consequences in EF of bilateral cavernous crush-injury (BNCI) type-1 diabetic (DM) rats compared to normal rats.
Material and Methods: 40 rats were divided in four groups: Sham, BNCI, DM and BNCI+DM. Sham rats underwent intraperitoneal injection (IP) of saline and after 1-month proceeded to a sham laparotomy. BNCI rats underwent to IP of saline and after 1-month to BNCI. DM rats underwent to IP of 60mg/kg-1 streptozotocin and after 1-month to a sham-laparotomy. BNCI +DM underwent to IP of 60mg/kg-1 STZ and after 1-month to BNCI. Blood glucose level (BGL) and weight were measured monthly. After 5-months from diabetes induction, all rats underwent to measurement of intracorporeal pressure (ICP) during CN-electrostimulation.
Results: BGL was higher (p<0.05) in DM and CCN+DM (>600mg/dl). After 5-months, DM and BNCI+DM rats showed lower weight compared to sham and BNCI rats (p<0.05). No significant difference was noted in ICP/MAP at all voltage between the sham and BNCI group. BNCI +DM showed lower ICP/MAP compared to all groups (p<0.05). DM Showed lower ICP/MAP compared to Sham and BNCI (p<0.05).
Conclusion: Normal Rats did not show any Erectile dysfunction 4-months after BNCI suggesting a spontaneous recovery of EF. BNCI in diabetic rats induced long-term erectile dysfunction.
The aim of this study is to evaluate the long-term consequences in EF of bilateral cavernous crush-injury (BNCI) type-1 diabetic (DM) rats compared to normal rats.
Material and Methods: 40 rats were divided in four groups: Sham, BNCI, DM and BNCI+DM. Sham rats underwent intraperitoneal injection (IP) of saline and after 1-month proceeded to a sham laparotomy. BNCI rats underwent to IP of saline and after 1-month to BNCI. DM rats underwent to IP of 60mg/kg-1 streptozotocin and after 1-month to a sham-laparotomy. BNCI +DM underwent to IP of 60mg/kg-1 STZ and after 1-month to BNCI. Blood glucose level (BGL) and weight were measured monthly. After 5-months from diabetes induction, all rats underwent to measurement of intracorporeal pressure (ICP) during CN-electrostimulation.
Results: BGL was higher (p<0.05) in DM and CCN+DM (>600mg/dl). After 5-months, DM and BNCI+DM rats showed lower weight compared to sham and BNCI rats (p<0.05). No significant difference was noted in ICP/MAP at all voltage between the sham and BNCI group. BNCI +DM showed lower ICP/MAP compared to all groups (p<0.05). DM Showed lower ICP/MAP compared to Sham and BNCI (p<0.05).
Conclusion: Normal Rats did not show any Erectile dysfunction 4-months after BNCI suggesting a spontaneous recovery of EF. BNCI in diabetic rats induced long-term erectile dysfunction.
Introduction: A recent statement from the European Society for Sexual Medicine has highlighted the limits of the rat model for nerve sparing radical prostatectomy like the use of young rats without comorbidities and the early evaluation of the erectile function (EF).
The aim of this study is to evaluate the long-term consequences in EF of bilateral cavernous crush-injury (BNCI) type-1 diabetic (DM) rats compared to normal rats.
Material and Methods: 40 rats were divided in four groups: Sham, BNCI, DM and BNCI+DM. Sham rats underwent intraperitoneal injection (IP) of saline and after 1-month proceeded to a sham laparotomy. BNCI rats underwent to IP of saline and after 1-month to BNCI. DM rats underwent to IP of 60mg/kg-1 streptozotocin and after 1-month to a sham-laparotomy. BNCI +DM underwent to IP of 60mg/kg-1 STZ and after 1-month to BNCI. Blood glucose level (BGL) and weight were measured monthly. After 5-months from diabetes induction, all rats underwent to measurement of intracorporeal pressure (ICP) during CN-electrostimulation.
Results: BGL was higher (p<0.05) in DM and CCN+DM (>600mg/dl). After 5-months, DM and BNCI+DM rats showed lower weight compared to sham and BNCI rats (p<0.05). No significant difference was noted in ICP/MAP at all voltage between the sham and BNCI group. BNCI +DM showed lower ICP/MAP compared to all groups (p<0.05). DM Showed lower ICP/MAP compared to Sham and BNCI (p<0.05).
Conclusion: Normal Rats did not show any Erectile dysfunction 4-months after BNCI suggesting a spontaneous recovery of EF. BNCI in diabetic rats induced long-term erectile dysfunction.
The aim of this study is to evaluate the long-term consequences in EF of bilateral cavernous crush-injury (BNCI) type-1 diabetic (DM) rats compared to normal rats.
Material and Methods: 40 rats were divided in four groups: Sham, BNCI, DM and BNCI+DM. Sham rats underwent intraperitoneal injection (IP) of saline and after 1-month proceeded to a sham laparotomy. BNCI rats underwent to IP of saline and after 1-month to BNCI. DM rats underwent to IP of 60mg/kg-1 streptozotocin and after 1-month to a sham-laparotomy. BNCI +DM underwent to IP of 60mg/kg-1 STZ and after 1-month to BNCI. Blood glucose level (BGL) and weight were measured monthly. After 5-months from diabetes induction, all rats underwent to measurement of intracorporeal pressure (ICP) during CN-electrostimulation.
Results: BGL was higher (p<0.05) in DM and CCN+DM (>600mg/dl). After 5-months, DM and BNCI+DM rats showed lower weight compared to sham and BNCI rats (p<0.05). No significant difference was noted in ICP/MAP at all voltage between the sham and BNCI group. BNCI +DM showed lower ICP/MAP compared to all groups (p<0.05). DM Showed lower ICP/MAP compared to Sham and BNCI (p<0.05).
Conclusion: Normal Rats did not show any Erectile dysfunction 4-months after BNCI suggesting a spontaneous recovery of EF. BNCI in diabetic rats induced long-term erectile dysfunction.
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